ABSTRACT
@#Studies on the potential effect of EMF exposure on permeability of the blood-brain barrier (BBB) in humans are virtually absent. This study was conducted to study the effect of EMF exposure on pericytes in brain tissues and its effect on oxidative stress level in the blood through total protein and malondialdehyde (MDA). About 16 male rats (Wistar) were used and divided into two groups which were negative control and treatment group. In negative control group, the animals were placed in a solenoid without any EMF exposure for 3 hours daily for 5 days. In the treatment group, the animals were placed in a solenoid with 0.3 mT EMF exposure for the same time duration. On day 3 and day 5, animals were sacrificed and the brain was removed for histological examination while on day 1, day 3 and day 5, the blood was collected for biochemistry analysis. Histological observation showed the presence of morphological changes in the brain tissues of rats that exposed to EMF. Statistical analysis showed that there is no significant decrease in total protein (p>0.05) between negative control group and treatment group. Meanwhile, MDA level in blood showed a significant increase in treatment group (p<0.05) as compared to the negative control group. The result obtained in this study, suggest that the exposure to EMF can cause changes to the morphology of pericytes in brain tissues, and can increase the MDA level in blood of rats.
ABSTRACT
This study was conducted to evaluate the effect of argan oil with the exposure of low frequency electromagnetic field (EMF)on open wound healing in mice. Eighteen male mice (20-40 g) were divided into three groups: phosphate buffer saline(PBS) as negative control, solcoseryl gel as positive control, and argan oil with the exposure of low frequency EMF, 1.2mT (treatment group). Full thickness wounds (4 mm diameter) were induced on the shaved dorsal of the mouse. All micewere sacrificed on day 12 after the final treatment. Macroscopic observation, wound contraction rate, histopathologyanalysis and total protein content were examined in this study. Results showed that wounds treated with argan oil andexposed to low frequency EMF has a significant increase in wound contraction rate (p < 0.05) and total protein content(p < 0.05). Moreover, histopathological analysis on the wound tissues displayed complete re-epithelization with thick anddense collagen fibers in the argan oil with low frequency EMF exposure treated group. In conclusion, topical treatment ofargan oil with low frequency EMF exposure yield a better healing progress and showed the ability to accelerate woundhealing.
ABSTRACT
The emergence of research about the biological effects of electromagnetic field (EMF) have growing concern among researchers. The aim of this study was to investigate the effects on the brain of rats periodically exposed to 0.1 mT EMF. Total 24 adult male Sprague Dawley rats were subdivided randomly to 4 groups: 2 control groups (C1 6 hours: 6 h/ day for 5 days; C2 20 hours: 20 h/day for 5 days) and 2 treatment groups which exposed to 0.1 mT EMF (T1 6 hours: 6 h/day for 5 days; T2 20 hours: 20 h/day for 5 days). A significant decrease in the pyramidal cell number was higher as the exposure duration to EMF was extended (T1, p<0.05; T2, p<0.001). The total numbers of pyramidal cells for T1 was 15.18 % lower than of the total found in C1; and in concurring to the pattern, the number of pyramidal cells in T2 was 33.54 % lower than the total in C2. Similarly, there was a significant decrease of the Purkinje cell number as the duration exposure to EMF, extended (T1, p<0.05; T2, p<0.001). The total numbers of Purkinje cells for T1 was 11.20 % lower than C1, in T2 was 16.19 % lower than in C2. There were significant differences between the thickness of granular layer and molecular layer in the control groups and treatment groups. We also report a significant difference in the levels of norepinephrine in T2, 10.71 % higher than C2. Cumulatively, these results suggested that exposure to EMF can exert negative effect on rats brains.